Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 79
Page 16-57
... activity from activities endogenous to mammalian cells . The following considerations are important when selecting a reporter gene ( Gorman et al . 1982a ) : • The enzymatic activity encoded by the prokaryotic gene must be readily ...
... activity from activities endogenous to mammalian cells . The following considerations are important when selecting a reporter gene ( Gorman et al . 1982a ) : • The enzymatic activity encoded by the prokaryotic gene must be readily ...
Page 16-58
... activity that can be assayed in the same cell extracts prepared for measurement of CAT activity . An enzyme frequently used for this purpose is E. coli B - galactosidase , which is expressed in transfected mammalian cells from a ...
... activity that can be assayed in the same cell extracts prepared for measurement of CAT activity . An enzyme frequently used for this purpose is E. coli B - galactosidase , which is expressed in transfected mammalian cells from a ...
Page 18-2
... activity or by employing assays that are independent of such activity . In a few cases , the protein of interest carries an enzymatic or other biological activity ( e.g. , the ability to bind a specific ligand ) that can be assayed in ...
... activity or by employing assays that are independent of such activity . In a few cases , the protein of interest carries an enzymatic or other biological activity ( e.g. , the ability to bind a specific ligand ) that can be assayed in ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
75 other sections not shown
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml