Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-62
... added from a stock solution just before the suspension buffer is used . The rate of inactivation in aqueous solution increases with pH and is faster at 25 ° C than at 4 ° C . The half - life of a 20 μм aqueous solution of PMSF is about ...
... added from a stock solution just before the suspension buffer is used . The rate of inactivation in aqueous solution increases with pH and is faster at 25 ° C than at 4 ° C . The half - life of a 20 μм aqueous solution of PMSF is about ...
Page 18-63
... added , just before the buffer is used , from a 1 м stock ( see Appendix B ) . 2. Place the sample in a boiling - water bath for 10 minutes . The sample should become highly viscous as a consequence of the release of high- molecular ...
... added , just before the buffer is used , from a 1 м stock ( see Appendix B ) . 2. Place the sample in a boiling - water bath for 10 minutes . The sample should become highly viscous as a consequence of the release of high- molecular ...
Page F-2
... added to the reaction depends on ( 1 ) the sequence of the protuding 5 ' termini at the end ( s ) of the DNA and ( 2 ) ... added to a final concentration of 5 mM before the appropriate dNTPs and the Klenow fragment of E. coli DNA ...
... added to the reaction depends on ( 1 ) the sequence of the protuding 5 ' termini at the end ( s ) of the DNA and ( 2 ) ... added to a final concentration of 5 mM before the appropriate dNTPs and the Klenow fragment of E. coli DNA ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml