Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xiii
... Agarose Gel Electrophoresis 6.8 PREPARATION AND EXAMINATION OF AGAROSE GELS 6.9 Preparation of an Agarose Gel 6.9 Minigels 6.14 Staining DNA in Agarose Gels 6.15 Decontamination of Ethidium Bromide Solutions 6.16 DECONTAMINATION OF ...
... Agarose Gel Electrophoresis 6.8 PREPARATION AND EXAMINATION OF AGAROSE GELS 6.9 Preparation of an Agarose Gel 6.9 Minigels 6.14 Staining DNA in Agarose Gels 6.15 Decontamination of Ethidium Bromide Solutions 6.16 DECONTAMINATION OF ...
Page A-4
... agarose agarose ( for plates ) 15 g / liter ( for top agar ) 7 g / liter ( for plates ) 15 g / liter ( for top agarose ) 7 g / liter Sterilize by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . When the medium is ...
... agarose agarose ( for plates ) 15 g / liter ( for top agar ) 7 g / liter ( for plates ) 15 g / liter ( for top agarose ) 7 g / liter Sterilize by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . When the medium is ...
Page F-6
... agarose gel . The gel must be cast with low - melting - temperature agarose and it must be poured and run in 1 × TAE electrophoresis buffer ( see Appendix B ) containing ethidium bromide ( 0.5 μg / ml ) rather than the conventional 0.5 ...
... agarose gel . The gel must be cast with low - melting - temperature agarose and it must be poured and run in 1 × TAE electrophoresis buffer ( see Appendix B ) containing ethidium bromide ( 0.5 μg / ml ) rather than the conventional 0.5 ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml