Molecular Cloning: A Laboratory Manual, Book 3 |
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Page B-10
... aliquots at -20 ° C . Dissolve 54 g of CaCl2 · 6H2O in 200 ml of pure H2O ( Milli - Q or equivalent ) . Sterilize the solu- tion by passage through a 0.22 - micron filter . Store in 1 - ml aliquots at -20 ° C . Dissolve 13.5 g of CaCl2 ...
... aliquots at -20 ° C . Dissolve 54 g of CaCl2 · 6H2O in 200 ml of pure H2O ( Milli - Q or equivalent ) . Sterilize the solu- tion by passage through a 0.22 - micron filter . Store in 1 - ml aliquots at -20 ° C . Dissolve 13.5 g of CaCl2 ...
Page B-11
... aliquots and store -20 ° C . Add 186.1 g of disodium ethylenediaminetetra- acetate 2H2O to 800 ml of H2O . Stir vigor- ously on a magnetic stirrer . Adjust the pH to 8.0 with NaOH ( ~ 20 g of NaOH pellets ) . Dis- pense into aliquots ...
... aliquots and store -20 ° C . Add 186.1 g of disodium ethylenediaminetetra- acetate 2H2O to 800 ml of H2O . Stir vigor- ously on a magnetic stirrer . Adjust the pH to 8.0 with NaOH ( ~ 20 g of NaOH pellets ) . Dis- pense into aliquots ...
Page B-13
... aliquots and sterilize by autoclaving . Dissolve 292.2 g of NaCl in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Dis- pense into aliquots and sterilize by auto- claving . Dissolve 100 g of electrophoresis - grade SDS in 900 ...
... aliquots and sterilize by autoclaving . Dissolve 292.2 g of NaCl in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Dis- pense into aliquots and sterilize by auto- claving . Dissolve 100 g of electrophoresis - grade SDS in 900 ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml