Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-19
... amount of target antigens . These assays include immunoprecipitation , western blotting , and solid - phase radioimmunoassays ( RIAs ) , which will be described in detail in the remain- der of this chapter . SOLID - PHASE ...
... amount of target antigens . These assays include immunoprecipitation , western blotting , and solid - phase radioimmunoassays ( RIAs ) , which will be described in detail in the remain- der of this chapter . SOLID - PHASE ...
Page 18-21
... amount of the radiolabeled second antibody that reacts with antigen- antibody complexes bound to a solid support varies from antigen to antigen . Typically , however , a saturating amount of protein antigen attached to an immobilized ...
... amount of the radiolabeled second antibody that reacts with antigen- antibody complexes bound to a solid support varies from antigen to antigen . Typically , however , a saturating amount of protein antigen attached to an immobilized ...
Page E-5
... amount of nucleic acid in a preparation . If the sample is pure ( i.e. , without significant amounts of contaminants such as proteins , phenol , agarose , or other nucleic acids ) , spectrophotometric measurement of the amount of ...
... amount of nucleic acid in a preparation . If the sample is pure ( i.e. , without significant amounts of contaminants such as proteins , phenol , agarose , or other nucleic acids ) , spectrophotometric measurement of the amount of ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml