Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-4
... ANTIGEN Aggregated protein is a much better immunogen than soluble , dispersed protein . Partial denaturation may help to increase immunogenicity but may yield antisera that fail to react with native ... ANTIGEN 18 4 AMOUNT OF ANTIGEN 18 4.
... ANTIGEN Aggregated protein is a much better immunogen than soluble , dispersed protein . Partial denaturation may help to increase immunogenicity but may yield antisera that fail to react with native ... ANTIGEN 18 4 AMOUNT OF ANTIGEN 18 4.
Page 18-5
... antigen . The length of these intervals varies from antigen to antigen and cannot be predicted in advance . The best procedure is to boost the animal when the titer of circulating antibody begins to drop . The amount of antigen used for ...
... antigen . The length of these intervals varies from antigen to antigen and cannot be predicted in advance . The best procedure is to boost the animal when the titer of circulating antibody begins to drop . The amount of antigen used for ...
Page 18-21
... antigen- antibody complexes bound to a solid support varies from antigen to antigen . Typically , however , a saturating amount of protein antigen attached to an immobilized first antibody might be expected to bind approximately 2 × 10 ...
... antigen- antibody complexes bound to a solid support varies from antigen to antigen . Typically , however , a saturating amount of protein antigen attached to an immobilized first antibody might be expected to bind approximately 2 × 10 ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml