Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 17
Page A-2
... autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . 4 4 Allow the solution to cool to 60 ° C or less , and then add 100 ml of a sterile solution of 0.17 м KH2PO4 , 0.72 м K2HPO4 . ( This solution is made by dissolving 2.31 ...
... autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . 4 4 Allow the solution to cool to 60 ° C or less , and then add 100 ml of a sterile solution of 0.17 м KH2PO4 , 0.72 м K2HPO4 . ( This solution is made by dissolving 2.31 ...
Page A-7
... autoclaving the solution for 15 minutes at 15 lb / sq . in . on liquid cycle . Per liter : · 1 м Tris Cl ( pH 7.5 ) 50 ml MgSO4 · 7H2O 2 g H2O to 1 liter Sterilize the buffer by autoclaving for 20 minutes at 15 lb / sq . in . on liquid ...
... autoclaving the solution for 15 minutes at 15 lb / sq . in . on liquid cycle . Per liter : · 1 м Tris Cl ( pH 7.5 ) 50 ml MgSO4 · 7H2O 2 g H2O to 1 liter Sterilize the buffer by autoclaving for 20 minutes at 15 lb / sq . in . on liquid ...
Page B-13
... autoclaving . Dissolve 292.2 g of NaCl in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Dis- pense into aliquots and sterilize by auto- claving . Dissolve 100 g of electrophoresis - grade SDS in 900 ml of H2O . Heat to 68 ° C ...
... autoclaving . Dissolve 292.2 g of NaCl in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Dis- pense into aliquots and sterilize by auto- claving . Dissolve 100 g of electrophoresis - grade SDS in 900 ml of H2O . Heat to 68 ° C ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml