Molecular Cloning: A Laboratory Manual, Book 3 |
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Page viii
... BACTERIOPHAGE VECTORS 2.55 Restriction and Modification 2.55 Amber Suppressors 2.55 Recombination Systems 2.55 ... PARTICLES 2.77 GLYCEROL STEP GRADIENT 2.78 EQUILIBRIUM CENTRIFUGATION IN CESIUM CHLORIDE 2.79 EXTRACTION OF BACTERIOPHAGE ...
... BACTERIOPHAGE VECTORS 2.55 Restriction and Modification 2.55 Amber Suppressors 2.55 Recombination Systems 2.55 ... PARTICLES 2.77 GLYCEROL STEP GRADIENT 2.78 EQUILIBRIUM CENTRIFUGATION IN CESIUM CHLORIDE 2.79 EXTRACTION OF BACTERIOPHAGE ...
Page G-8
... molecules into bacteriophage particles in vitro . Proc . Natl . Acad . Sci . 74 : 3259 . Huang , W.M. and I.R. Lehman . 1972. On the exonuclease activity of phage T4 deoxyribonucleic acid polymerase . J. Biol . Chem . 247 : 3139 . ISCO ...
... molecules into bacteriophage particles in vitro . Proc . Natl . Acad . Sci . 74 : 3259 . Huang , W.M. and I.R. Lehman . 1972. On the exonuclease activity of phage T4 deoxyribonucleic acid polymerase . J. Biol . Chem . 247 : 3139 . ISCO ...
Page 1-24
... bacteriophage M13 , 4.25 for amplification of plasmid DNA , 1.21 for ... proteins , 18.53 lon , ATP - dependent protease in E. coli , 2.57 Long terminal repeat ... particles , 4.4 and Bluescript vectors , 1.20 cloning ligation , 4.35-4.38 ...
... bacteriophage M13 , 4.25 for amplification of plasmid DNA , 1.21 for ... proteins , 18.53 lon , ATP - dependent protease in E. coli , 2.57 Long terminal repeat ... particles , 4.4 and Bluescript vectors , 1.20 cloning ligation , 4.35-4.38 ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml