Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-17
... BINDING SITES In addition to the bacterial promoter , the second major factor required to express cloned DNA in E. coli is an efficient ribosome - binding site . In E. coli , the ribosome - binding site includes an initiation codon ...
... BINDING SITES In addition to the bacterial promoter , the second major factor required to express cloned DNA in E. coli is an efficient ribosome - binding site . In E. coli , the ribosome - binding site includes an initiation codon ...
Page 18-11
... binding sites for protein A. Because protein A itself has four potential sites for binding to IgG ( Sjödahl 1977 ) , it is possible to form multimeric complexes of the two types of protein . Not all immunoglobulins bind to protein A ...
... binding sites for protein A. Because protein A itself has four potential sites for binding to IgG ( Sjödahl 1977 ) , it is possible to form multimeric complexes of the two types of protein . Not all immunoglobulins bind to protein A ...
Page 18-69
... bind to the nitrocellulose filter , so can proteins in the immunological reagents used for probing . The sensitivity of western blotting depends on reducing this background of nonspecific binding by blocking potential binding sites with ...
... bind to the nitrocellulose filter , so can proteins in the immunological reagents used for probing . The sensitivity of western blotting depends on reducing this background of nonspecific binding by blocking potential binding sites with ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml