Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 15
Page 18-23
... bovine serum albumin serves as a blocking agent by binding to sites on the PVC plate that are not occupied by immunoglobulin molecules . The antibody - coated plates may be stored for several days at 4 ° C if the wells are completely ...
... bovine serum albumin serves as a blocking agent by binding to sites on the PVC plate that are not occupied by immunoglobulin molecules . The antibody - coated plates may be stored for several days at 4 ° C if the wells are completely ...
Page 18-24
... bovine serum albumin through the column . Then wash the column with 5 volumes of PBS lacking bovine serum albumin . When the column is ready , wrap a piece of Parafilm around the bottom of the pipette . This prevents the column from ...
... bovine serum albumin through the column . Then wash the column with 5 volumes of PBS lacking bovine serum albumin . When the column is ready , wrap a piece of Parafilm around the bottom of the pipette . This prevents the column from ...
Page B-26
... bovine serum albumin ( Fraction V ; Sigma ) 1 mм ẞ - mercaptoethanol This buffer is used with restriction enzymes . 10 × Klenow buffer 0.5 M Tris Cl ( pH 7.6 ) 0.1 M MgCl2 The Klenow fragment of E. coli DNA polymerase I also works in a ...
... bovine serum albumin ( Fraction V ; Sigma ) 1 mм ẞ - mercaptoethanol This buffer is used with restriction enzymes . 10 × Klenow buffer 0.5 M Tris Cl ( pH 7.6 ) 0.1 M MgCl2 The Klenow fragment of E. coli DNA polymerase I also works in a ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
75 other sections not shown
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml