Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-58
... carried out with amounts of extract that contain a defined amount of B - galactosidase activity . Finally , both enzymatic assays can be carried out in a constant volume of extract , and the results can then be normalized to a defined ...
... carried out with amounts of extract that contain a defined amount of B - galactosidase activity . Finally , both enzymatic assays can be carried out in a constant volume of extract , and the results can then be normalized to a defined ...
Page 18-45
... carried out with RIPA buffer . The third wash is carried out with 10 mM Tris · Cl ( pH 7.5 ) , 0.1 % NP - 40 . 5. Incubate the resuspended beads for 20 minutes at 4 ° C on a rocking platform . This allows time for the wash buffer to ...
... carried out with RIPA buffer . The third wash is carried out with 10 mM Tris · Cl ( pH 7.5 ) , 0.1 % NP - 40 . 5. Incubate the resuspended beads for 20 minutes at 4 ° C on a rocking platform . This allows time for the wash buffer to ...
Page 18-64
... carried out by direct electrophoretic transfer of proteins from the gel to a nitrocellulose filter ( Burnette 1981 ) . Two types of electrophoresis apparatuses are available for electroblotting . In the older type , one side of the gel ...
... carried out by direct electrophoretic transfer of proteins from the gel to a nitrocellulose filter ( Burnette 1981 ) . Two types of electrophoresis apparatuses are available for electroblotting . In the older type , one side of the gel ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml