Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-28
... cell lines can be obtained that carry up to 1000 copies of the dhfr gene ( for review , see Schimke 1984 , 1988 ; Stark and Wahl 1984 ) . The DNA unit that is amplified under selective conditions varies from cell line to cell line but ...
... cell lines can be obtained that carry up to 1000 copies of the dhfr gene ( for review , see Schimke 1984 , 1988 ; Stark and Wahl 1984 ) . The DNA unit that is amplified under selective conditions varies from cell line to cell line but ...
Page 16-30
... cells . Calcium phos- phate - mediated transfection is also used to establish cell lines that carry integrated copies of the foreign DNA , which are usually arranged in head - to - tail tandem arrays . · • Polybrene . The polycation ...
... cells . Calcium phos- phate - mediated transfection is also used to establish cell lines that carry integrated copies of the foreign DNA , which are usually arranged in head - to - tail tandem arrays . · • Polybrene . The polycation ...
Page 16-28
... cell lines can be obtained that carry up to 1000 copies of the dhfr gene ( for review , see Schimke 1984 , 1988 ; Stark and Wahl 1984 ) . The DNA unit that is amplified under selective conditions varies from cell line to cell line but ...
... cell lines can be obtained that carry up to 1000 copies of the dhfr gene ( for review , see Schimke 1984 , 1988 ; Stark and Wahl 1984 ) . The DNA unit that is amplified under selective conditions varies from cell line to cell line but ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml