Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 98
Page 17-43
... coli . Gene 42 : 175 . Marston , F.A.O. 1987. The purification of eukaryotic polypeptides expressed in Escherichia coli . In DNA cloning : A practical approach ( ed . D.M. Glover ) , vol . 3 , p . 59. IRL Press , Oxford . Marston ...
... coli . Gene 42 : 175 . Marston , F.A.O. 1987. The purification of eukaryotic polypeptides expressed in Escherichia coli . In DNA cloning : A practical approach ( ed . D.M. Glover ) , vol . 3 , p . 59. IRL Press , Oxford . Marston ...
Page 1-20
... coli strain , 4.15 , A.13 JM105 , E. coli strain genotype and uses , A.10 use in propagation of bacteriophage M13 vectors , 4.14 , A.13 JM106 , E. coli strain , 4.15 , A.13 JM107 , E. coli strain genotype and uses , A.10 use in ...
... coli strain , 4.15 , A.13 JM105 , E. coli strain genotype and uses , A.10 use in propagation of bacteriophage M13 vectors , 4.14 , A.13 JM106 , E. coli strain , 4.15 , A.13 JM107 , E. coli strain genotype and uses , A.10 use in ...
Page 1-37
... coli strain , genotype and uses , 17.13 , A.11 recA , genetic marker in E. coli host strain , 2.57 , 3.6 , 4.13 RecA protein , characteristics and use , 5.88 recA strains of E. coli , 3.6 , 3.34 . See also E. coli , strain list recB ...
... coli strain , genotype and uses , 17.13 , A.11 recA , genetic marker in E. coli host strain , 2.57 , 3.6 , 4.13 RecA protein , characteristics and use , 5.88 recA strains of E. coli , 3.6 , 3.34 . See also E. coli , strain list recB ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml