Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-12
... column , and then wash the column with 10 column volumes of 100 mM Tris Cl ( pH 8.0 ) followed by 10 volumes of 10 mM Tris Cl ( pH 8.0 ) . 4. Add 1 column volume of 100 mm glycine ( pH 3.0 ) . Begin collecting 500 - μl fractions of the ...
... column , and then wash the column with 10 column volumes of 100 mM Tris Cl ( pH 8.0 ) followed by 10 volumes of 10 mM Tris Cl ( pH 8.0 ) . 4. Add 1 column volume of 100 mm glycine ( pH 3.0 ) . Begin collecting 500 - μl fractions of the ...
Page E-32
... column . Bio - Gel HTP has a capacity of 100-200 μg of native DNA per milliliter of bed volume . 4. Prepare the hydroxyapatite columns in disposable 3 - ml plastic syringes as follows : a . Remove the barrel from the syringe . b . Using ...
... column . Bio - Gel HTP has a capacity of 100-200 μg of native DNA per milliliter of bed volume . 4. Prepare the hydroxyapatite columns in disposable 3 - ml plastic syringes as follows : a . Remove the barrel from the syringe . b . Using ...
Page E-37
... column . Spun - column chromatography is particularly useful when separat- ing labeled DNA from radioactive precursors ( e.g. , in nick - translation reac- tions ) . However , it is also used extensively for other purposes , for example ...
... column . Spun - column chromatography is particularly useful when separat- ing labeled DNA from radioactive precursors ( e.g. , in nick - translation reac- tions ) . However , it is also used extensively for other purposes , for example ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml