Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-17
... complete mammalian transcription unit ( see pages 16.5-16.9 ) and a selectable marker ( see pages 16.9-16.15 ) are inserted into a prokaryotic plasmid . The resulting vector is then amplified in bacteria before being transfected into ...
... complete mammalian transcription unit ( see pages 16.5-16.9 ) and a selectable marker ( see pages 16.9-16.15 ) are inserted into a prokaryotic plasmid . The resulting vector is then amplified in bacteria before being transfected into ...
Page 18-6
... complete adjuvant . Use the emulsion immediately . Inject 50 μl of the emulsified antigen - adjuvant subcutaneously at each of four sites . Four to 6 weeks later , boost the mice with an intraperitoneal injection of 100 μl of the ...
... complete adjuvant . Use the emulsion immediately . Inject 50 μl of the emulsified antigen - adjuvant subcutaneously at each of four sites . Four to 6 weeks later , boost the mice with an intraperitoneal injection of 100 μl of the ...
Page 18-28
... complete medium , prewarmed to 37 ° C . The cells should then be incubated for the appropriate time at 37 ° C in complete , prewarmed medium contain- ing five times the normal amount of nonradioactive methionine ( or both methionine and ...
... complete medium , prewarmed to 37 ° C . The cells should then be incubated for the appropriate time at 37 ° C in complete , prewarmed medium contain- ing five times the normal amount of nonradioactive methionine ( or both methionine and ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml