Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 10
Page 18-57
... deionized water . Photographic reducing solution can be made as follows ( Switzer et al . 1979 ) : a . Solution A : Dissolve 37 g of NaCl and 37 g of CuSO4 in 850 ml of deionized H2O . Add concentrated NH4OH until a deep blue ...
... deionized water . Photographic reducing solution can be made as follows ( Switzer et al . 1979 ) : a . Solution A : Dissolve 37 g of NaCl and 37 g of CuSO4 in 850 ml of deionized H2O . Add concentrated NH4OH until a deep blue ...
Page A-2
... deionized H2O . After the salts have dissolved , adjust the volume of the solution to 100 ml with deionized H2O and sterilize by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . ) SOB Medium Per liter : To 950 ml of ...
... deionized H2O . After the salts have dissolved , adjust the volume of the solution to 100 ml with deionized H2O and sterilize by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . ) SOB Medium Per liter : To 950 ml of ...
Page A-3
... deionized H2O , add : bacto - tryptone 16 g bacto - yeast extract 10 g 5 g NaCl Shake until the solutes have dissolved . Adjust the pH to 7.0 with 5 N NaOH . Adjust the volume of the solution to 1 liter with deionized H2O . Sterilize by ...
... deionized H2O , add : bacto - tryptone 16 g bacto - yeast extract 10 g 5 g NaCl Shake until the solutes have dissolved . Adjust the pH to 7.0 with 5 N NaOH . Adjust the volume of the solution to 1 liter with deionized H2O . Sterilize by ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml