Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-3
... denatured forms of the protein . These are raised against fully denatured antigens and can be either monoclonal or polyclonal . Factors Affecting Immune Response Although there is no way to guarantee the production of particular types ...
... denatured forms of the protein . These are raised against fully denatured antigens and can be either monoclonal or polyclonal . Factors Affecting Immune Response Although there is no way to guarantee the production of particular types ...
Page 18-4
... denatured proteins ( e.g. , proteins that have been extensively boiled , reduced , and alkylated ) often yield antisera that react exclusively or preferentially with denatured protein . Many immunology textbooks state that denatured pro ...
... denatured proteins ( e.g. , proteins that have been extensively boiled , reduced , and alkylated ) often yield antisera that react exclusively or preferentially with denatured protein . Many immunology textbooks state that denatured pro ...
Page 18-60
... denatured or native ) . Consequently , not all monoclonal antibodies are suitable for use as probes in western blots , where the target proteins are thoroughly denatured . Polyclon- al antisera , on the other hand , are undefined ...
... denatured or native ) . Consequently , not all monoclonal antibodies are suitable for use as probes in western blots , where the target proteins are thoroughly denatured . Polyclon- al antisera , on the other hand , are undefined ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml