Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-38
... described on pages 16.33-16.34 , steps 1 and 3 . 2. While the precipitate is forming , collect the exponentially growing cells by centrifugation at 800g for 5 minutes at 4 ° C . Discard the supernatant , and resuspend the cell pellet in ...
... described on pages 16.33-16.34 , steps 1 and 3 . 2. While the precipitate is forming , collect the exponentially growing cells by centrifugation at 800g for 5 minutes at 4 ° C . Discard the supernatant , and resuspend the cell pellet in ...
Page 18-80
... described on pages 18.44-18.46 , and detailed and standardized procedures for electrophoresis in SDS - poly- acrylamide gels are described on pages 18.47-18.54 . Notes i . For analysis by SDS - polyacrylamide gel electrophoresis ...
... described on pages 18.44-18.46 , and detailed and standardized procedures for electrophoresis in SDS - poly- acrylamide gels are described on pages 18.47-18.54 . Notes i . For analysis by SDS - polyacrylamide gel electrophoresis ...
Page E-26
... described in Chapter 6 , page 6.45 , and dried onto Whatman 3MM paper using a commercial gel dryer . b . Sequencing gels containing 35S should be fixed as described in Chapter 13 , page 13.56 , and dried onto Whatman 3MM paper using a ...
... described in Chapter 6 , page 6.45 , and dried onto Whatman 3MM paper using a commercial gel dryer . b . Sequencing gels containing 35S should be fixed as described in Chapter 13 , page 13.56 , and dried onto Whatman 3MM paper using a ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml