Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 47
Page 16-71
... desired gene . Transformation with genomic DNA results in uptake by the recipient cells of considerably more DNA than the DNA of the desired gene . Frequently , 0.01-0.1 % of the genome equivalent of DNA may be taken up by recipient ...
... desired gene . Transformation with genomic DNA results in uptake by the recipient cells of considerably more DNA than the DNA of the desired gene . Frequently , 0.01-0.1 % of the genome equivalent of DNA may be taken up by recipient ...
Page E-16
... desired volume of aqueous phase is achieved . Note Because 2 - butanol extraction does not remove salt , the salt concentration increases in proportion to the reduction in the volume of the solution . The nucleic acid can be transferred ...
... desired volume of aqueous phase is achieved . Note Because 2 - butanol extraction does not remove salt , the salt concentration increases in proportion to the reduction in the volume of the solution . The nucleic acid can be transferred ...
Page F-6
... desired restriction fragment of foreign DNA and the appropriate segment of plasmid DNA . In the protocol given below ( adapted from Struhl 1985 by S. Michaelis , pers . comm . ) , ligation of plasmid and foreign DNAs is carried out ...
... desired restriction fragment of foreign DNA and the appropriate segment of plasmid DNA . In the protocol given below ( adapted from Struhl 1985 by S. Michaelis , pers . comm . ) , ligation of plasmid and foreign DNAs is carried out ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
75 other sections not shown
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml