Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-21
... dilution of antigen that will give half - maximal binding of radiolabeled antibody . In this case , it is essential that the dilutions span a wide range from background to maximal binding . For accurate quantitation of antigen , it is ...
... dilution of antigen that will give half - maximal binding of radiolabeled antibody . In this case , it is essential that the dilutions span a wide range from background to maximal binding . For accurate quantitation of antigen , it is ...
Page 18-23
... dilutions in blocking buffer of the antigen that is to be assayed . Add 50 μl of each dilution to each of two wells . Cover the plate and incubate it for 2 hours at room temperature in a humidified atmo- sphere or for 8 hours at 4 ° C ...
... dilutions in blocking buffer of the antigen that is to be assayed . Add 50 μl of each dilution to each of two wells . Cover the plate and incubate it for 2 hours at room temperature in a humidified atmo- sphere or for 8 hours at 4 ° C ...
Page B-23
... dilution of the concentrated stock ) for agarose gel electrophoresis . However , a working solution of 0.5 × provides more than enough buffering power , and almost all agarose gel electrophoresis is now carried out with a 1:10 dilution ...
... dilution of the concentrated stock ) for agarose gel electrophoresis . However , a working solution of 0.5 × provides more than enough buffering power , and almost all agarose gel electrophoresis is now carried out with a 1:10 dilution ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml