Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 95
Page 16-5
... et al . 1981 ; Gething and Sambrook 1981 ; Rigby 1982 , 1983 ; Doyle et al . 1985 ; Sambrook et al . 1986 ) , vaccinia virus ( Mackett et al . 1985 ; Moss 1985 ; Fuerst et al . 1986 , 1987 ) , adenovirus ( Solnick 1981 ; Thummel et al ...
... et al . 1981 ; Gething and Sambrook 1981 ; Rigby 1982 , 1983 ; Doyle et al . 1985 ; Sambrook et al . 1986 ) , vaccinia virus ( Mackett et al . 1985 ; Moss 1985 ; Fuerst et al . 1986 , 1987 ) , adenovirus ( Solnick 1981 ; Thummel et al ...
Page 16-25
... et al . 1982 ; Zinn et al . 1983 ; Sambrook et al . 1985 ) , but not in all ( DiMaio et al . 1984 ) , this fragment appears to carry an activity that enhances the ability of BPV vectors containing only the 69 % transform- ing fragment ...
... et al . 1982 ; Zinn et al . 1983 ; Sambrook et al . 1985 ) , but not in all ( DiMaio et al . 1984 ) , this fragment appears to carry an activity that enhances the ability of BPV vectors containing only the 69 % transform- ing fragment ...
Page 16-54
... et al . 1982 ) . The method has been used to introduce DNA into a variety of animal cells ( Neumann et al . 1982 ; Wong and Neumann 1982 ; Potter et al . 1984 ; Sugden et al . 1985 ; Toneguzzo et al . 1986 ; Tur - Kaspa et al . 1986 ) ...
... et al . 1982 ) . The method has been used to introduce DNA into a variety of animal cells ( Neumann et al . 1982 ; Wong and Neumann 1982 ; Potter et al . 1984 ; Sugden et al . 1985 ; Toneguzzo et al . 1986 ; Tur - Kaspa et al . 1986 ) ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml