Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 32
Page 17-22
... ethanol at 0 ° C . DNA by centrifugation at 12,000g for 10 minutes at 0 ° C . supernatant , and wash the pellet with 70 % ethanol . Redissolve the DNA in 20 μl of the appropriate 1 x restriction enzyme buffer . 4. Cleave the DNA at site ...
... ethanol at 0 ° C . DNA by centrifugation at 12,000g for 10 minutes at 0 ° C . supernatant , and wash the pellet with 70 % ethanol . Redissolve the DNA in 20 μl of the appropriate 1 x restriction enzyme buffer . 4. Cleave the DNA at site ...
Page 18-83
... ethanol . After storage for 30 minutes at -20 ° C , collect the RNA by centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . Remove as much of the ethanol as possible by gentle aspiration , and leave the open tube on the ...
... ethanol . After storage for 30 minutes at -20 ° C , collect the RNA by centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . Remove as much of the ethanol as possible by gentle aspiration , and leave the open tube on the ...
Page E-16
... ethanol . 1. Measure the volume of the nucleic acid solution and add an equal volume of 2 - butanol . Mix the ... ethanol as described on pages E.12 - E.14 . DRYING DOWN 32P - LABELED NUCLEOTIDES FROM MLXTURES OF ETHANOL E.16 Commonly ...
... ethanol . 1. Measure the volume of the nucleic acid solution and add an equal volume of 2 - butanol . Mix the ... ethanol as described on pages E.12 - E.14 . DRYING DOWN 32P - LABELED NUCLEOTIDES FROM MLXTURES OF ETHANOL E.16 Commonly ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml