Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xxvii
... Expression in Mammalian Cells 16.68 Cloning by Expression of cDNA Clones 16.69 Expression Cloning of Genomic DNA 16.70 References 16.73 17 Expression of Cloned Genes in Escherichia coli Production of Fusion Proteins 17.3 Vector Systems ...
... Expression in Mammalian Cells 16.68 Cloning by Expression of cDNA Clones 16.69 Expression Cloning of Genomic DNA 16.70 References 16.73 17 Expression of Cloned Genes in Escherichia coli Production of Fusion Proteins 17.3 Vector Systems ...
Page 16-68
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Cloning by Expression in Mammalian Cells In addition to the uses of expression in mammalian cells described above , mammalian expression can be an important tool in the identification ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Cloning by Expression in Mammalian Cells In addition to the uses of expression in mammalian cells described above , mammalian expression can be an important tool in the identification ...
Page 16-69
... expression is to prepare cDNA from mRNA containing the expressed gene of interest and to insert this cDNA into an expression vector for a given target cell type . The most commonly used recipient cell type has been COS cells ( see page ...
... expression is to prepare cDNA from mRNA containing the expressed gene of interest and to insert this cDNA into an expression vector for a given target cell type . The most commonly used recipient cell type has been COS cells ( see page ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml