Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 85
Page 16-58
... extract is measured and the CAT assay is then carried out with amounts of extract that contain a defined amount of B - galactosidase activity . Finally , both enzymatic assays can be carried out in a constant volume of extract , and the ...
... extract is measured and the CAT assay is then carried out with amounts of extract that contain a defined amount of B - galactosidase activity . Finally , both enzymatic assays can be carried out in a constant volume of extract , and the ...
Page 16-60
... extract at -20 ° C . 4. Incubate the 50 - μl aliquot of the extract for 10 minutes at 65 ° C to inactivate deacetylases . If the extract is cloudy or opaque at this stage , remove the particulate material by centrifugation at 12,000g ...
... extract at -20 ° C . 4. Incubate the 50 - μl aliquot of the extract for 10 minutes at 65 ° C to inactivate deacetylases . If the extract is cloudy or opaque at this stage , remove the particulate material by centrifugation at 12,000g ...
Page 16-63
... Extracts of transfected cells , prepared as described on pages 16.59 and 16.60 , steps 1-3 ( except reserve 30 μl of the extract for the assay ) , are assayed as follows : 1. Prepare CAT reaction mixture 2 : 8 mm chloramphenicol cell ...
... Extracts of transfected cells , prepared as described on pages 16.59 and 16.60 , steps 1-3 ( except reserve 30 μl of the extract for the assay ) , are assayed as follows : 1. Prepare CAT reaction mixture 2 : 8 mm chloramphenicol cell ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
75 other sections not shown
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml