Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 38
Page 16-49
... final concentration of 250 μg / ml . Continue incubation overnight . See Chapter 1 , pages 1.21 and 1.33 , for a discussion of the use of chloramphenicol in the amplification of plasmids . 2. Recover the bacterial cells from 50 ml of ...
... final concentration of 250 μg / ml . Continue incubation overnight . See Chapter 1 , pages 1.21 and 1.33 , for a discussion of the use of chloramphenicol in the amplification of plasmids . 2. Recover the bacterial cells from 50 ml of ...
Page 18-45
... final concentration of 0.5 M. For the second wash , standard NET - gel buffer is supplemented with SDS ( final concentration 0.1 % ) . The third wash is carried out with 10 mM Tris Cl ( pH 7.5 ) , 0.1 % NP - 40 . • RIPA buffer ( see ...
... final concentration of 0.5 M. For the second wash , standard NET - gel buffer is supplemented with SDS ( final concentration 0.1 % ) . The third wash is carried out with 10 mM Tris Cl ( pH 7.5 ) , 0.1 % NP - 40 . • RIPA buffer ( see ...
Page E-12
... final solution is 400 μl or less , precipitation may be carried out in a single microfuge tube . Larger volumes can be divided among several microfuge tubes , or the DNA can be precipitated and centrifuged in tubes that will fit in a ...
... final solution is 400 μl or less , precipitation may be carried out in a single microfuge tube . Larger volumes can be divided among several microfuge tubes , or the DNA can be precipitated and centrifuged in tubes that will fit in a ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml