Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-18
... fragment extending from the second codon to a restriction site 3 ' of the cloned gene is made by one of the methods given below . SYNTHESIS OF DNA ENCODING THE AMINO TERMINUS 1. Synthesize a double - stranded DNA adapter extending from ...
... fragment extending from the second codon to a restriction site 3 ' of the cloned gene is made by one of the methods given below . SYNTHESIS OF DNA ENCODING THE AMINO TERMINUS 1. Synthesize a double - stranded DNA adapter extending from ...
Page 17-25
... fragment prepared by one of the methods described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described ...
... fragment prepared by one of the methods described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described ...
Page F-2
... fragment of E. coli DNA polymerase I GAATTOH 3 ' СТТААp5 ' On the other hand , all four dNTPs are required to fill recessed termini created by HindIII : dATP dTTP dCTP dGTP „ А он 3 ' TTCGAр5 ' Klenow fragment of E. coli DNA polymerase ...
... fragment of E. coli DNA polymerase I GAATTOH 3 ' СТТААp5 ' On the other hand , all four dNTPs are required to fill recessed termini created by HindIII : dATP dTTP dCTP dGTP „ А он 3 ' TTCGAр5 ' Klenow fragment of E. coli DNA polymerase ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml