Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 69
Page B-15
... hybridization Benton / Davis hybridization all Southern hybridizations other than single - copy dot blots 1 × BLOTTO ( Bovine Lacto Transfer Technique Optimizer ; Johnson et al . 1984 ) is 5 % nonfat dried milk dissolved in water ...
... hybridization Benton / Davis hybridization all Southern hybridizations other than single - copy dot blots 1 × BLOTTO ( Bovine Lacto Transfer Technique Optimizer ; Johnson et al . 1984 ) is 5 % nonfat dried milk dissolved in water ...
Page 1-18
... hybridization probes , 11.52-11.53 labeling , 11.15-11.16 3H , isotopic data , B.8 H HAT ( hypoxanthine , aminopterin , and thymidine ) medium , use as selec- tive agent , 16.9 , 16.14 HB101 , E. coli strain genotype and uses , A.10 ...
... hybridization probes , 11.52-11.53 labeling , 11.15-11.16 3H , isotopic data , B.8 H HAT ( hypoxanthine , aminopterin , and thymidine ) medium , use as selec- tive agent , 16.9 , 16.14 HB101 , E. coli strain genotype and uses , A.10 ...
Page 1-29
... hybridization and autoradiography , 7.52 staining RNA , 7.42 , 7.44-7.45 , 7.51 transfer of RNA to solid supports ... Hybridization activated , for western blotting , 18.64 capillary transfer of nucleic acids to supports , 7.49-7.50 ...
... hybridization and autoradiography , 7.52 staining RNA , 7.42 , 7.44-7.45 , 7.51 transfer of RNA to solid supports ... Hybridization activated , for western blotting , 18.64 capillary transfer of nucleic acids to supports , 7.49-7.50 ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml