Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 37
Page 18-43
... lysate should be treated with an irrelevant monoclonal antibody that binds to staphylococcal protein A. 1. To a sample of lysate , add either 0.05 volume of preimmune serum , or 1 volume of irrelevant hybridoma tissue culture ...
... lysate should be treated with an irrelevant monoclonal antibody that binds to staphylococcal protein A. 1. To a sample of lysate , add either 0.05 volume of preimmune serum , or 1 volume of irrelevant hybridoma tissue culture ...
Page 18-79
... lysate RNA Incubate for 1 hour at 30 ° C . 2 μl 2 μ 1 μ . 8 μ . 10 με 2 μ . Thaw reticulocyte lysates gently ( hand - heat ) , and return unused portions to -70 ° C or to liquid nitrogen as soon as possible . Repeated freezing and ...
... lysate RNA Incubate for 1 hour at 30 ° C . 2 μl 2 μ 1 μ . 8 μ . 10 με 2 μ . Thaw reticulocyte lysates gently ( hand - heat ) , and return unused portions to -70 ° C or to liquid nitrogen as soon as possible . Repeated freezing and ...
Page 18-80
... lysate to 2 mм . When next preparing a batch of lysate , use a higher concentration of micrococcal nuclease ( rather than treating for a longer time ) , and make sure that the lysate is equilibrated to 20 ° C during treatment with the ...
... lysate to 2 mм . When next preparing a batch of lysate , use a higher concentration of micrococcal nuclease ( rather than treating for a longer time ) , and make sure that the lysate is equilibrated to 20 ° C during treatment with the ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
75 other sections not shown
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml