Molecular Cloning: A Laboratory Manual, Book 3 |
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Page ix
... LYSOGENS 2.100 PROTOCOL I : PACKAGING IN VITRO USING TWO EXTRACTS 2.104 PROTOCOL II : PREPARATION OF PACKAGING EXTRACTS FROM ONE LYSOGEN 2.105 PROTOCOL II : PACKAGING IN VITRO USING ONE EXTRACT 2.107 Identification and Analysis of ...
... LYSOGENS 2.100 PROTOCOL I : PACKAGING IN VITRO USING TWO EXTRACTS 2.104 PROTOCOL II : PREPARATION OF PACKAGING EXTRACTS FROM ONE LYSOGEN 2.105 PROTOCOL II : PACKAGING IN VITRO USING ONE EXTRACT 2.107 Identification and Analysis of ...
Page A-9
... lysogen used to prepare packaging extracts ( Hohn and Murray 1977 ; Hohn 1979 ) . A strain employed for high - level ... lysogeny mutation suppresses plaque formation by cl bacteriophages but allows plaque formation by recombinant cl ...
... lysogen used to prepare packaging extracts ( Hohn and Murray 1977 ; Hohn 1979 ) . A strain employed for high - level ... lysogeny mutation suppresses plaque formation by cl bacteriophages but allows plaque formation by recombinant cl ...
Page A-11
... lysogenic strain used as a host for plasmids containing the bacteriophage A P1 promoter ( Nagai and Thøgersen 1984 ) ... lysogen used to prepare packaging extracts ( Rosenberg 1985 ) . TAP90 TG1 TG2 XL1 - Blue XS101 XS127 Y1089 Y1090hsdR ...
... lysogenic strain used as a host for plasmids containing the bacteriophage A P1 promoter ( Nagai and Thøgersen 1984 ) ... lysogen used to prepare packaging extracts ( Rosenberg 1985 ) . TAP90 TG1 TG2 XL1 - Blue XS101 XS127 Y1089 Y1090hsdR ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml