Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-9
... mRNAs transcribed from mammalian expression vectors ( Shaw and Kamen 1986 ) . Although similar motifs have been found in analogous locations within mRNAs encoding a variety of growth factors and oncogenes , relatively little is known ...
... mRNAs transcribed from mammalian expression vectors ( Shaw and Kamen 1986 ) . Although similar motifs have been found in analogous locations within mRNAs encoding a variety of growth factors and oncogenes , relatively little is known ...
Page 18-79
... mRNA to mRNA . The amount of magnesium acetate required to achieve the maximum efficiency of transla- tion should be ... mRNAs . Up to 10 μg of total cytoplasmic RNA or 0.2 μg of poly ( A ) * mRNA or RNA synthesized in vitro can be added ...
... mRNA to mRNA . The amount of magnesium acetate required to achieve the maximum efficiency of transla- tion should be ... mRNAs . Up to 10 μg of total cytoplasmic RNA or 0.2 μg of poly ( A ) * mRNA or RNA synthesized in vitro can be added ...
Page 18-81
... mRNA can affect its stability and the efficiency of translation . All natural eukaryotic mRNAs and many viral mRNAs have a structure at their 5 ' termini consisting of a 7 - methylguanosine residue linked by a triphosphate bridge to the ...
... mRNA can affect its stability and the efficiency of translation . All natural eukaryotic mRNAs and many viral mRNAs have a structure at their 5 ' termini consisting of a 7 - methylguanosine residue linked by a triphosphate bridge to the ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml