Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 48
Page 18-8
... mg / ml . KLH ( Calbiochem no . 374817 ) is supplied at a concentration of 100 mg / ml in 50 % glycerol . KLH should be stored at 4 ° C . The pH of PBS is adjusted to pH 6.0 with 3 N HCl . 2. Transfer 1 ml of the KLH solution to a clean 15 ...
... mg / ml . KLH ( Calbiochem no . 374817 ) is supplied at a concentration of 100 mg / ml in 50 % glycerol . KLH should be stored at 4 ° C . The pH of PBS is adjusted to pH 6.0 with 3 N HCl . 2. Transfer 1 ml of the KLH solution to a clean 15 ...
Page 18-12
... mg and 20 mg of pure IgG . Protein A - Sepharose is very expensive and should be used sparingly . For the preparation of IgG for most laboratory purposes , 1 - ml columns are poured and run in pasteur pipettes plugged with sterile glass ...
... mg and 20 mg of pure IgG . Protein A - Sepharose is very expensive and should be used sparingly . For the preparation of IgG for most laboratory purposes , 1 - ml columns are poured and run in pasteur pipettes plugged with sterile glass ...
Page 18-31
... ml trypsin Stock solution 10 mg / ml in 0.01 M HEPES ( pH 8.0 ) chymotrypsin plasmin Leupeptins plasmin ( supplied as a 3 : 1 trypsin chymotrypsin pepsin 1-2 μg / ml 10 mg / ml in water mixture of propionyl papain cathepsins A and D and ...
... ml trypsin Stock solution 10 mg / ml in 0.01 M HEPES ( pH 8.0 ) chymotrypsin plasmin Leupeptins plasmin ( supplied as a 3 : 1 trypsin chymotrypsin pepsin 1-2 μg / ml 10 mg / ml in water mixture of propionyl papain cathepsins A and D and ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml