Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 31
Page 18-35
... microfuge tube . This is best done with a red - hot hypodermic needle ( 23 gauge ) . Place the microfuge tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube ...
... microfuge tube . This is best done with a red - hot hypodermic needle ( 23 gauge ) . Place the microfuge tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube ...
Page 18-39
... microfuge tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube at 2000g for 2 minutes at 4 ° C . 11. Recover the bottom microfuge tube , which should contain ...
... microfuge tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube at 2000g for 2 minutes at 4 ° C . 11. Recover the bottom microfuge tube , which should contain ...
Page E-12
... tube . Larger volumes can be divided among several microfuge tubes , or the DNA can be precipitated and centrifuged in tubes that will fit in a medium - speed centrifuge or ultracentrifuge . 3. Mix the solution well ... MICROFUGE TUBES E 12.
... tube . Larger volumes can be divided among several microfuge tubes , or the DNA can be precipitated and centrifuged in tubes that will fit in a medium - speed centrifuge or ultracentrifuge . 3. Mix the solution well ... MICROFUGE TUBES E 12.
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml