Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 76
Page 16-60
... minutes at 4 ° C in a microfuge . Transfer the supernatant to a fresh microfuge tube . Reserve 50 μl for the CAT assay , and store the remainder of the extract at -20 ° C . 4. Incubate the 50 - μl aliquot of the extract for 10 minutes ...
... minutes at 4 ° C in a microfuge . Transfer the supernatant to a fresh microfuge tube . Reserve 50 μl for the CAT assay , and store the remainder of the extract at -20 ° C . 4. Incubate the 50 - μl aliquot of the extract for 10 minutes ...
Page 17-32
... 4. To obtain high levels of transcription from the phoA promoter , grow an ... C in a microfuge to pellet the cells . Discard the supernatant . b . Suspend ... minutes . Lysozyme will not work efficiently if the pH of the solution is less ...
... 4. To obtain high levels of transcription from the phoA promoter , grow an ... C in a microfuge to pellet the cells . Discard the supernatant . b . Suspend ... minutes . Lysozyme will not work efficiently if the pH of the solution is less ...
Page 18-83
... C ( bacteriophages T3 and T7 DNA - dependent RNA polymerases ) or 40 ° C ... minutes at 37 ° C . 6. Add 100 μl of RNAase - free H2O and purify the RNA by ... 4 ° C in a microfuge . Redissolve the RNA in 100 μl of RNAse - free H2O and ...
... C ( bacteriophages T3 and T7 DNA - dependent RNA polymerases ) or 40 ° C ... minutes at 37 ° C . 6. Add 100 μl of RNAase - free H2O and purify the RNA by ... 4 ° C in a microfuge . Redissolve the RNA in 100 μl of RNAse - free H2O and ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
75 other sections not shown
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml