Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-3
... monoclonal antibodies that are pan - specific are not uncommon . • Antibodies that react only with epitopes specific to the native form of the target protein . Antibodies of this type are typically monoclonal and recog- nize a given ...
... monoclonal antibodies that are pan - specific are not uncommon . • Antibodies that react only with epitopes specific to the native form of the target protein . Antibodies of this type are typically monoclonal and recog- nize a given ...
Page 18-7
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Monoclonal Antibodies The introduction of cell hybridization to generate myeloma - like cell lines that secrete monoclonal antibodies with desired specificities has greatly expanded the ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Monoclonal Antibodies The introduction of cell hybridization to generate myeloma - like cell lines that secrete monoclonal antibodies with desired specificities has greatly expanded the ...
Page 18-60
... antibodies that react specifically with an- tigenic epitopes displayed by the target protein attached to the solid ... monoclonal antibodies are suitable for use as probes in western blots , where the target proteins are thoroughly ...
... antibodies that react specifically with an- tigenic epitopes displayed by the target protein attached to the solid ... monoclonal antibodies are suitable for use as probes in western blots , where the target proteins are thoroughly ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml