Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 39
Page 18-17
... nitrocellulose filter as described on pages 18.64-18.66 . 2. After transfer , incubate the filter in blocking buffer for 1 hour at room temperature . Use 0.25 ml of blocking buffer for each square centimeter of nitrocellulose filter ...
... nitrocellulose filter as described on pages 18.64-18.66 . 2. After transfer , incubate the filter in blocking buffer for 1 hour at room temperature . Use 0.25 ml of blocking buffer for each square centimeter of nitrocellulose filter ...
Page 18-64
... nitrocellulose filter ( Burnette 1981 ) . Two types of electrophoresis apparatuses are available for electroblotting . In the older type , one side of the gel is placed in contact with a piece of nitrocellulose filter . The gel and its ...
... nitrocellulose filter ( Burnette 1981 ) . Two types of electrophoresis apparatuses are available for electroblotting . In the older type , one side of the gel is placed in contact with a piece of nitrocellulose filter . The gel and its ...
Page 18-67
... nitrocellulose filter are then marked with pencil or indelible ink . If the western blot is to be probed with radiolabeled antibody or radiolabeled protein A , the proteins immobilized on the nitrocellulose filter may be stained with ...
... nitrocellulose filter are then marked with pencil or indelible ink . If the western blot is to be probed with radiolabeled antibody or radiolabeled protein A , the proteins immobilized on the nitrocellulose filter may be stained with ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
75 other sections not shown
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml