Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-63
... phase to a fresh tube . Add 100 μl of ice - cold ethyl acetate to the original tube ( the aqueous phase ) . Vortex vigorously and centrifuge again as in step 3. Transfer 100 μl of the organic phase to the tube containing 80 μl of ethyl ...
... phase to a fresh tube . Add 100 μl of ice - cold ethyl acetate to the original tube ( the aqueous phase ) . Vortex vigorously and centrifuge again as in step 3. Transfer 100 μl of the organic phase to the tube containing 80 μl of ethyl ...
Page E-3
... phase forms the upper phase . However , if the aqueous phase is dense because of salt ( > 0.5 M ) or sucrose ( > 10 % ) , it will form the lower phase . The organic phase is easily identifiable because of the yellow color contributed by ...
... phase forms the upper phase . However , if the aqueous phase is dense because of salt ( > 0.5 M ) or sucrose ( > 10 % ) , it will form the lower phase . The organic phase is easily identifiable because of the yellow color contributed by ...
Page E-16
... phase . By carrying out several cycles of extraction , the volume of a nucleic acid solution can be reduced ... phase until an aqueous phase ( which should contain the nucleic acid ) reappears . 2. Centrifuge the solution at room ...
... phase . By carrying out several cycles of extraction , the volume of a nucleic acid solution can be reduced ... phase until an aqueous phase ( which should contain the nucleic acid ) reappears . 2. Centrifuge the solution at room ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml