Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 24
Page viii
... PLAQUE PURIFICATION OF BACTERIOPHAGE X 2.60 Preparation of Plating Bacteria 2.60 Plating Bacteriophage λ 2.61 Picking Bacteriophage λ Plaques 2.63 PREPARING STOCKS OF BACTERIOPHAGE À FROM SINGLE PLAQUES 2.64 Plate Lysate Stocks 2.64 ...
... PLAQUE PURIFICATION OF BACTERIOPHAGE X 2.60 Preparation of Plating Bacteria 2.60 Plating Bacteriophage λ 2.61 Picking Bacteriophage λ Plaques 2.63 PREPARING STOCKS OF BACTERIOPHAGE À FROM SINGLE PLAQUES 2.64 Plate Lysate Stocks 2.64 ...
Page xi
... Plaques 4.24 PREPARING STOCKS OF BACTERIOPHAGE M13 FROM SINGLE PLAQUES 4.25 Liquid Cultures 4.25 PREPARATION OF BACTERIOPHAGE M13 DNA 4.26 Small - scale Preparations of the Replicative Form of Bacteriophage M13 DNA 4.26 Small - scale ...
... Plaques 4.24 PREPARING STOCKS OF BACTERIOPHAGE M13 FROM SINGLE PLAQUES 4.25 Liquid Cultures 4.25 PREPARATION OF BACTERIOPHAGE M13 DNA 4.26 Small - scale Preparations of the Replicative Form of Bacteriophage M13 DNA 4.26 Small - scale ...
Page 1-18
... plaques , 2.108- 2.117 of bacteriophage M13 plaques , 4.41 northern , of RNA , 7.37 , 7.39-7.52 prehybridization solution , 1.101-1.102 , 9.52-9.53 , 11.56 of radiolabeled probes to immobilized nucleic acids , 9.47-9.57 . See also ...
... plaques , 2.108- 2.117 of bacteriophage M13 plaques , 4.41 northern , of RNA , 7.37 , 7.39-7.52 prehybridization solution , 1.101-1.102 , 9.52-9.53 , 11.56 of radiolabeled probes to immobilized nucleic acids , 9.47-9.57 . See also ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml