Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 33
Page 16-61
... plate in the chromatography chamber , close the chamber , and allow the solvent front to move approximately 75 % of the distance to the top of the plate . 14. Remove the TLC plate from the tank and allow it to dry at room temperature ...
... plate in the chromatography chamber , close the chamber , and allow the solvent front to move approximately 75 % of the distance to the top of the plate . 14. Remove the TLC plate from the tank and allow it to dry at room temperature ...
Page 18-23
... plate that are not occupied by immunoglobulin molecules . The antibody - coated plates may be stored for several ... plate and incubate it for 2 hours at room temperature in a humidified atmo- sphere or for 8 hours at 4 ° C . 8. Wash the ...
... plate that are not occupied by immunoglobulin molecules . The antibody - coated plates may be stored for several ... plate and incubate it for 2 hours at room temperature in a humidified atmo- sphere or for 8 hours at 4 ° C . 8. Wash the ...
Page A-4
... Plates can then be poured directly from the flask ; allow about 30-35 ml of medium per 90 - mm plate . To remove bubbles from medium in the plate , flame the surface of the medium with a bunsen burner before the agar or agarose hardens ...
... Plates can then be poured directly from the flask ; allow about 30-35 ml of medium per 90 - mm plate . To remove bubbles from medium in the plate , flame the surface of the medium with a bunsen burner before the agar or agarose hardens ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml