Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 11
Page 18-20
... polyclonal antibody or a monoclonal antibody of different specificity . How- ever , in some cases , it may be possible to use the same polyclonal antibody for both parts of the assay . If suitable antibodies are available , this is the ...
... polyclonal antibody or a monoclonal antibody of different specificity . How- ever , in some cases , it may be possible to use the same polyclonal antibody for both parts of the assay . If suitable antibodies are available , this is the ...
Page 18-42
... polyclonal antisera raised in rats and sheep , and some polyclonal antisera raised in mice , hamsters , cows , and horses . Although some murine monoclonal antibodies bind efficiently to protein A , rat mono- clonal antibodies rarely do ...
... polyclonal antisera raised in rats and sheep , and some polyclonal antisera raised in mice , hamsters , cows , and horses . Although some murine monoclonal antibodies bind efficiently to protein A , rat mono- clonal antibodies rarely do ...
Page 18-60
... polyclonal antiserum will detect different antigenic epitopes of an immobil- ized , denatured target protein . Although there is an obvious danger that comes from using undefined reagents to assay a target protein that may also be ...
... polyclonal antiserum will detect different antigenic epitopes of an immobil- ized , denatured target protein . Although there is an obvious danger that comes from using undefined reagents to assay a target protein that may also be ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml