Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-34
... precipitate should form At the end of the incubation , pipette the mature up and down once to resuspend the precipitate . A commonly used alternative procedure 28 to and a fine mure of CaC , and DNA w the 2x HBS solution For example ...
... precipitate should form At the end of the incubation , pipette the mature up and down once to resuspend the precipitate . A commonly used alternative procedure 28 to and a fine mure of CaC , and DNA w the 2x HBS solution For example ...
Page 16-39
... precipitate to form during this time . 4. Add the CaCl2 / DNA / BES - buffered saline solution dropwise to the dishes of cells ... precipitate to a fine precipitate occurs Expression of Cloned Genes in Cultured Mammalian Cells 16.39.
... precipitate to form during this time . 4. Add the CaCl2 / DNA / BES - buffered saline solution dropwise to the dishes of cells ... precipitate to a fine precipitate occurs Expression of Cloned Genes in Cultured Mammalian Cells 16.39.
Page 16-34
... precipitate should form . At the end of the incubation , pipette the mixture up and down once to resuspend the precipitate . A commonly used alternative procedure is to add a dilute mixture of CaCl , and DNA to the 2 × HBS solution ...
... precipitate should form . At the end of the incubation , pipette the mixture up and down once to resuspend the precipitate . A commonly used alternative procedure is to add a dilute mixture of CaCl , and DNA to the 2 × HBS solution ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml