Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-2
... proteins . These antibodies have been used ( 1 ) to purify proteins by immunoaffinity chromatography ; ( 2 ) in diagnostic assays to quantitate the levels of protein ; and ( 3 ) to localize the proteins in organisms , tissues , and ...
... proteins . These antibodies have been used ( 1 ) to purify proteins by immunoaffinity chromatography ; ( 2 ) in diagnostic assays to quantitate the levels of protein ; and ( 3 ) to localize the proteins in organisms , tissues , and ...
Page 17-29
... protein not have an amino - terminal methionine , which is coded for by the ATG in these vectors . The amino ... Protein That Can Be Cleaved by a Protease or Cyanogen Bromide The cloned gene can be expressed as part of a fusion protein ...
... protein not have an amino - terminal methionine , which is coded for by the ATG in these vectors . The amino ... Protein That Can Be Cleaved by a Protease or Cyanogen Bromide The cloned gene can be expressed as part of a fusion protein ...
Page 18-2
... protein that are synthesized in small - scale mammalian cultures . ( 2 ) The host cells may themselves express an endoge- nous protein that either displays the same biological activity as the foreign protein or interferes with it . ( 3 ) ...
... protein that are synthesized in small - scale mammalian cultures . ( 2 ) The host cells may themselves express an endoge- nous protein that either displays the same biological activity as the foreign protein or interferes with it . ( 3 ) ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml