Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 59
Page xviii
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
Page 18-21
... radiolabeled antibody . Antibodies radiolabeled with 125I as described later in this chapter ( see pages 18.24- 18.25 ) have an approximate specific activity of 5 × 106 cpm / μg . The amount of the radiolabeled second antibody that ...
... radiolabeled antibody . Antibodies radiolabeled with 125I as described later in this chapter ( see pages 18.24- 18.25 ) have an approximate specific activity of 5 × 106 cpm / μg . The amount of the radiolabeled second antibody that ...
Page 18-26
... radiolabeled protein . When coupled with SDS - polyacrylamide gel electrophoresis , the technique is ideal for analysis of the synthesis and processing of foreign antigens expressed in prokaryotic and eukaryotic hosts or in in vitro ...
... radiolabeled protein . When coupled with SDS - polyacrylamide gel electrophoresis , the technique is ideal for analysis of the synthesis and processing of foreign antigens expressed in prokaryotic and eukaryotic hosts or in in vitro ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
75 other sections not shown
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml