Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-63
... reaction , dilute the 14C - labeled acetyl coenzyme A tenfold into a solution of unlabeled acetyl coenzyme A ( 0.5 mM ) . The concentrations of the reagents in the final reaction mixture are : 2.3 mM chloramphenicol , 0.11 M Tris Cl ...
... reaction , dilute the 14C - labeled acetyl coenzyme A tenfold into a solution of unlabeled acetyl coenzyme A ( 0.5 mM ) . The concentrations of the reagents in the final reaction mixture are : 2.3 mM chloramphenicol , 0.11 M Tris Cl ...
Page 16-64
... reaction mixture is overlaid with water - immiscible scintillation fluid . Acetylated forms of chloramphenicol produced by the activity of CAT in the aqueous phase diffuse rapidly into the scintillation fluid Neumann et al . 1987 ...
... reaction mixture is overlaid with water - immiscible scintillation fluid . Acetylated forms of chloramphenicol produced by the activity of CAT in the aqueous phase diffuse rapidly into the scintillation fluid Neumann et al . 1987 ...
Page F-8
... reactions . In the first reaction , blunt - ended double - stranded linkers are attached to the DNA of interest ( which must also be blunt - ended ) . Because the synthetic linkers are very small ( 8-12 bp ) , it is relatively easy to ...
... reactions . In the first reaction , blunt - ended double - stranded linkers are attached to the DNA of interest ( which must also be blunt - ended ) . Because the synthetic linkers are very small ( 8-12 bp ) , it is relatively easy to ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml