Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-7
... sequences that facili- tate the propagation of the vector in bacteria and one or more eukaryotic transcription units that are expressed only in eukaryotic cells . The eukaryotic transcription unit consists of noncoding sequences and ...
... sequences that facili- tate the propagation of the vector in bacteria and one or more eukaryotic transcription units that are expressed only in eukaryotic cells . The eukaryotic transcription unit consists of noncoding sequences and ...
Page 16-9
... Sequences within the 3 ' noncoding regions of eukaryotic genes may play a role in mRNA stability . For example , the presence of an AU - rich sequence , derived originally from the 3 ' noncoding region of granulocyte - macrophage colony ...
... Sequences within the 3 ' noncoding regions of eukaryotic genes may play a role in mRNA stability . For example , the presence of an AU - rich sequence , derived originally from the 3 ' noncoding region of granulocyte - macrophage colony ...
Page 16-15
... Sequences DNAs encoding the foreign protein of interest are usually cloned as cDNAs that lack all of the controlling elements required for expression in mam- malian cells but may contain ancillary sequences introduced during the ...
... Sequences DNAs encoding the foreign protein of interest are usually cloned as cDNAs that lack all of the controlling elements required for expression in mam- malian cells but may contain ancillary sequences introduced during the ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml