Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xviii
... DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE ... stranded DNA Probes 10.6 NICK TRANSLATION OF ...
... DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE ... stranded DNA Probes 10.6 NICK TRANSLATION OF ...
Page xxv
... Single - stranded Target DNA 15.53 Design and Selection of Mutagenic Oligonucleotides 15.54 Hybridization of Oligonucleotides to the Template DNA and Primer Extension 15.57 Transfection of E. coli and Screening for Mutants 15.59 ...
... Single - stranded Target DNA 15.53 Design and Selection of Mutagenic Oligonucleotides 15.54 Hybridization of Oligonucleotides to the Template DNA and Primer Extension 15.57 Transfection of E. coli and Screening for Mutants 15.59 ...
Page E-30
... double - stranded DNA . Neoprene gasket microfuge tube circulating water heater Lucite box FIGURE E.30 Commonly Used Techniques in Molecular Cloning SEPARATION OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA HYDROXYAPATITE CHROMATOGRAPHY E 30.
... double - stranded DNA . Neoprene gasket microfuge tube circulating water heater Lucite box FIGURE E.30 Commonly Used Techniques in Molecular Cloning SEPARATION OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA HYDROXYAPATITE CHROMATOGRAPHY E 30.
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml