Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 13
Page 18-32
... sodium azide 0.1 % SDS 100 μg / ml phenylmethylsulfonyl fluoride ( PMSF ) 1 μg / ml aprotinin 1 % Nonidet P - 40 ( NP - 40 ) 0.5 % sodium deoxycholate Single - detergent lysis buffer 50 mM Tris Cl ( pH 8.0 ) 150 mM NaCl 0.02 % sodium ...
... sodium azide 0.1 % SDS 100 μg / ml phenylmethylsulfonyl fluoride ( PMSF ) 1 μg / ml aprotinin 1 % Nonidet P - 40 ( NP - 40 ) 0.5 % sodium deoxycholate Single - detergent lysis buffer 50 mM Tris Cl ( pH 8.0 ) 150 mM NaCl 0.02 % sodium ...
Page 18-44
... sodium azide The gelatin is diluted from a 2.5 % ( w / v ) stock that has been solubilized and sterilized by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . The stock solution can be melted just before use by brief ...
... sodium azide The gelatin is diluted from a 2.5 % ( w / v ) stock that has been solubilized and sterilized by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . The stock solution can be melted just before use by brief ...
Page B-15
... sodium azide . It should be stored at 4 ° C and diluted 25 - fold into prehybridization buffer before use . BLOTTO should not be used in combination with high concentrations of SDS , which will cause the milk proteins to precipitate ...
... sodium azide . It should be stored at 4 ° C and diluted 25 - fold into prehybridization buffer before use . BLOTTO should not be used in combination with high concentrations of SDS , which will cause the milk proteins to precipitate ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml