Molecular Cloning: A Laboratory Manual, Book 3 |
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Page A-3
... sterilized by autoclaving for 15 minutes at 15 lb / sq . in . on liquid cycle . The MgSO , and CaCl , solutions should be prepared separately , sterilized by autoclaving , and added after diluting the 5 × M9 salts to 1 liter with sterile ...
... sterilized by autoclaving for 15 minutes at 15 lb / sq . in . on liquid cycle . The MgSO , and CaCl , solutions should be prepared separately , sterilized by autoclaving , and added after diluting the 5 × M9 salts to 1 liter with sterile ...
Page A-5
... sterile glycerol ( sterilized by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle ) . Vortex the culture to ensure that the glycerol is evenly dispersed . Transfer the culture to a labeled storage tube equipped with a ...
... sterile glycerol ( sterilized by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle ) . Vortex the culture to ensure that the glycerol is evenly dispersed . Transfer the culture to a labeled storage tube equipped with a ...
Page A-7
... sterile 20 % maltose solution for every 100 ml of medium . Make up a sterile 20 % stock solution of maltose as follows : maltose H2O to 100 ml 20 g Sterilize the solution by filtration through a 0.22 - micron filter . Store the sterile ...
... sterile 20 % maltose solution for every 100 ml of medium . Make up a sterile 20 % stock solution of maltose as follows : maltose H2O to 100 ml 20 g Sterilize the solution by filtration through a 0.22 - micron filter . Store the sterile ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml