Molecular Cloning: A Laboratory Manual, Book 3 |
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Page B-9
A Laboratory Manual Joseph Sambrook, Tom Maniatis. STOCK SOLUTIONS AND BLOCKING AGENTS TABLE B.7 Preparation of Commonly Used Stock Solutions Solution Method of preparation Comments TABLE B.7 ( continued ) Solution Method of preparation ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. STOCK SOLUTIONS AND BLOCKING AGENTS TABLE B.7 Preparation of Commonly Used Stock Solutions Solution Method of preparation Comments TABLE B.7 ( continued ) Solution Method of preparation ...
Page G-13
... stock solutions , 6.39 , 13.47 , B.9 / urea for sequencing , 13.48 Actinomycin D inhibits second - strand cDNA synthesis , 5.54 , 8.56 precautions for laboratory safety , 5.54 , B.9 stock solution , B.9 ADA . See Adenosine deaminase ...
... stock solutions , 6.39 , 13.47 , B.9 / urea for sequencing , 13.48 Actinomycin D inhibits second - strand cDNA synthesis , 5.54 , 8.56 precautions for laboratory safety , 5.54 , B.9 stock solution , B.9 ADA . See Adenosine deaminase ...
Page 1-42
... stock solution , B.13 use in precipitating nucleic acids , E.10 - E.15 Sodium dodecyl sulfate . See SDS Sodium hydroxide ( NaOH ) common commercial strengths , B.2 pH values of stock solutions , B.3 Sodium lauryl sarcosinate , in solutions ...
... stock solution , B.13 use in precipitating nucleic acids , E.10 - E.15 Sodium dodecyl sulfate . See SDS Sodium hydroxide ( NaOH ) common commercial strengths , B.2 pH values of stock solutions , B.3 Sodium lauryl sarcosinate , in solutions ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml