Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 54
Page 18-10
... stored in small aliquots that are labeled with the name of the animal and the date of the bleed . Polyclonal antisera are best stored undiluted , whereas monoclonal antibodies should be stored at concentrations of > 1 mg / ml in ...
... stored in small aliquots that are labeled with the name of the animal and the date of the bleed . Polyclonal antisera are best stored undiluted , whereas monoclonal antibodies should be stored at concentrations of > 1 mg / ml in ...
Page 18-55
... stored indefinitely in water in a sealed plastic bag without any diminution in the intensity of staining . However , fixed polyacrylamide gels stored in water will swell and may distort during storage . To avoid this problem , store ...
... stored indefinitely in water in a sealed plastic bag without any diminution in the intensity of staining . However , fixed polyacrylamide gels stored in water will swell and may distort during storage . To avoid this problem , store ...
Page B-15
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
75 other sections not shown
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml