Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 69
Page 17-36
... strains defective in termination of transcription ( rho ) or altered in RNA metabolism ( pnp , rna ) to increase the amount of functional RNA . Also try several different strains of E. coli because expression levels vary in different ...
... strains defective in termination of transcription ( rho ) or altered in RNA metabolism ( pnp , rna ) to increase the amount of functional RNA . Also try several different strains of E. coli because expression levels vary in different ...
Page A-13
... Strains JM106 and JM108 are identical to JM107 and JM109 , respectively , except that they do not carry an F ' episome . These strains will not support the growth of bacteriophage M13 but may be used to propagate plasmids . However ...
... Strains JM106 and JM108 are identical to JM107 and JM109 , respectively , except that they do not carry an F ' episome . These strains will not support the growth of bacteriophage M13 but may be used to propagate plasmids . However ...
Page I-14
... strains dam , 2.57 hfl ( high frequency of lysogenization ) , 2.11-2.12 , 2.57 hsdM , 2.57 hsdR , 2.57 , 4.13 hsdS , 2.57 lacla , 2.57 , 4.12 A ( lac - proAB ) , deletion of lac operon , 4.12 lacZAM15 , 2.57 , 4.12 lon , 2.57 mcrB ...
... strains dam , 2.57 hfl ( high frequency of lysogenization ) , 2.11-2.12 , 2.57 hsdM , 2.57 hsdR , 2.57 , 4.13 hsdS , 2.57 lacla , 2.57 , 4.12 A ( lac - proAB ) , deletion of lac operon , 4.12 lacZAM15 , 2.57 , 4.12 lon , 2.57 mcrB ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml